RS-FISH: precise, interactive, fast, and scalable FISH spot detection
Dec 1, 2022·,,,,,,,,,,·
1 min read
Ella Bahry
Laura Breimann
Marwan Zouinkhi
Leo Epstein
Klim Kolyvanov
Nicholas Mamrak
Benjamin King
Xi Long
Kyle I. S. Harrington
Timothée Lionnet
Stephan Preibisch
Abstract
RS-FISH is an accurate, fast, and user-friendly software for spot detection in two- and three-dimensional images. It offers interactive parameter tuning and scales to large datasets and image volumes of cleared or expanded samples using distributed processing on workstations, clusters, or the cloud. RS-FISH maintains high detection accuracy and low localization error across a wide range of signal-to-noise ratios, making it suitable for single-molecule FISH, spatial transcriptomics, or spatial genomics applications.
Type
Publication
Nature Methods, 19(12)
RS-FISH is an accurate, fast, and user-friendly software for spot detection in two- and three-dimensional images. It offers interactive parameter tuning and scales to large datasets and image volumes of cleared or expanded samples using distributed processing on workstations, clusters, or the cloud. RS-FISH maintains high detection accuracy and low localization error across a wide range of signal-to-noise ratios, making it suitable for single-molecule FISH, spatial transcriptomics, or spatial genomics applications.